Johnson City has an extensive network of bike lanes and trails that offer a fun and eco-friendly way to explore the city's natural beauty and surrounding areas. The city has several parks, including Buffalo Mountain Park, which offers some of the most scenic and challenging mountain biking trails in the region.

The Tweetsie Trail is a popular biking trail in the area, which runs from Johnson City to Elizabethton. The trail is relatively flat and is ideal for all skill levels. Another popular destination for bikers is the Virginia Creeper Trail, which is a 34-mile trail that runs from Abingdon, VA to Damascus, VA. The trail offers breathtaking scenery and is suitable for riders of all ages and skill levels.

In addition to the trails and bike lanes, there are several bike rental and repair shops in the area. These shops offer a range of services, including bike rentals, repairs, and accessories.